This has touched my heart and soul. For thirteen years I have felt this but could never find the correct words. Thank you for the words and validating our loss. Time does help, the bad days aren’t as often but it is always there, one mundane word, song, thought will sink you once again into the darkness of reality. I realized after a time that this is how the new normal is and just as the young people of today say, Roll with it, the sadness. They mattered, will always matter, so have that cry, tears are cleansing.
To each and every one of you, I am sorry for the loss. My heart aches for all of who have ben forced to have a membership in this club.
You may want to identify certain types of equipment by vendor name and brand or category (., ultracentrifuge vs. prep centrifuge), particularly if they are not commonly found in most labs. It is appropriate to report, parenthetically, the source (vendor) and catalog number for reagents used, ., " ....poly-L-lysine (Sigma #1309) ." When using a method described in another published source, you can save time and words by providing the relevant citation to the source. Always make sure to describe any modifications you have made of a standard or published method.